Csfv e2 phosphatase

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August undefined, 2024

WebRecombinded CSFV E2 protein; classical swine fever virus (strain Alfort) (CSFV) (Hog cholera virus) 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. … WebApr 9, 2024 · E2 is the major structural glycoprotein of the classical swine fever virus (CSFV). E2 has been shown to be involved in important virus functions such as replication and virulence in swine. Using the yeast two-hybrid system, we previously identified several host proteins specifically interacting with CSFV E2. Here, we analyze the protein …

Expression and purification of classical swine fever virus E2 …

WebMar 29, 2024 · Classical swine fever virus (CSFV) E2 protein, the major virus structural glycoprotein, is an essential component of the viral envelope. E2 is involved in virus absorption, induction of a protective immune … WebJan 8, 2014 · E2 is one of the envelope glycoproteins of pestiviruses, including classical swine fever virus (CSFV) and bovine viral diarrhea virus (BVDV). E2 is involved in several critical functions, including virus entry … can a limited liability company have partners

Identification of classical swine fever virus protein E2 as a target ...

WebThe recombinant CSFV E2 sequence was also fused to a 6× histidine tag to facilitate purification and the endoplasmic reticulum (ER) retrieval HDEL tag to allow accumulation of the protein in the endoplasmic reticulum. All genes were codon optimized for plant expression using the Nicotiana tabaccum codon usage table and synthesized by Eurofins ... WebDec 12, 2024 · The E2 protein in classical swine fever (CSF) virus (CSFV) is the major virus structural glycoprotein and is an essential component of the viral particle. E2 … WebThe CSFV E2 protein has been widely used as a major antigen for antibody detection. It is important to improve the affinity between the E2 protein and CSFV antibodies to improve the performance of the detection method. In this study, a recombinant E2 extracellular protein (amino acids 1–331) with a native homodimer conformation and high ... can a man over 60 get a woman pregnant

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WebTo further confirm the infection of CSFV, a pair of specific primers were designed for detection of CSFV E2 genes in the collected immune organs (tonsil, thymus, spleen and inguinal lymph node) through reverse transcription (RT)-PCR method. Simultaneously, E2 expression in the collected immune organs was analyzed by IHC method exploiting a ... WebMar 1, 2008 · The microtiter plate was coated with total soluble proteins from the wild-type and transformed plants and the known CSFV E2 antigen at 4 °C overnight. The wells … can a lymph node ruptureWebMar 8, 2024 · Author summary Classical swine fever virus (CSFV) is a highly pathogenic RNA virus belonging to Flaviviridae family that can cause deadly classical swine fever (CSF) among pigs. In this study, we identified ADAM17 as a binding partner for CSFV envelope protein E2 using biochemical approaches. Knockout of ADAM17 rendered permissive … can a number be a divisor of itself

"Webfever virus (CSFV), the causative agent of this disease, is an enveloped, positive-strand RNA virus belonging to the genus Pestivirus in the family Flaviviridae. The envelope of CSFV contains three glycoproteins, Erns, E1, and E2.2) Among these, E2 is the most immuno-genic, and is a virulence determinant.3) Post-transla- " - Csfv e2 phosphatase

Csfv e2 phosphatase

Generation and immunogenicity analysis of ... - Virology Journal

WebSep 1, 2024 · Objective To develop a simple method for efficient expression of classical swine fever virus (CSFV) E2 protein. Results The pFastBac HT B vector (pFastHTB-M1) was modified by adding a melittin signal peptide sequence. The E2 gene fragment without the transmembrane region was cloned into pFastHTB-M1. The modified vector has clear … WebMay 1, 2014 · Monoclonal anti-E2 CSFV antibody (diluted 1:1000) and rabbit polyclonal serum anti-E rns CSFV (diluted 1:250) were used as primary antibodies. Anti-mouse and anti-rabbit alkaline phosphatase (AP)-conjugated antibodies were used as secondary antibodies (diluted 1:2000). Antigen-antibody complexes were detected using NBT and …

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WebJun 24, 2024 · CSFV structural protein E2 was observed colocalizing with CALCOCO2/NDP52 in PK-15 cells. CALCOCO2/NDP52 silencing also decreased the autophagic markers CD63, LC3 and BECN1; however, colocalization between LC3 and ubiquitin was also decreased. ... Skeletal and kidney-enriched inositol phosphatase. … WebDec 5, 2005 · CSFV E1 (B) was detected with an E1 specific rabbit antiserum, and CSFV E2 (C) was detected with mAb WH303. ... slides were incubated with alkaline-phosphatase-conjugated, goat anti-mouse antibody for 20 min at room temperature. Acknowledgments. We thank Aniko Zsak, Adriene Lakowitz, and the PIADC animal care staff for excellent …

WebMar 29, 2024 · DOE PAGES ® Journal Article: Interaction of Structural Glycoprotein E2 of Classical Swine Fever Virus with Protein Phosphatase 1 Catalytic Subunit Beta … WebSep 1, 2024 · Objective To develop a simple method for efficient expression of classical swine fever virus (CSFV) E2 protein. Results The pFastBac HT B vector (pFastHTB-M1) …

WebCSFV E2, which is not identical to the one in BVDV, was shown to interact with a host cell receptor (15), presumably CD46. (Overall, the amino acid sequences of BVDV and CSFV E2 are 65% identical.) The most similar structure to domain II is the ﬁrst Ig domain of receptor for advanced glycation end products WebOct 10, 2024 · The cells were incubated CSFV E2 antibody (JBT, 9011) at 4°C overnight (in dilute E2 protein antibody with PBS at a ratio of 1:200 (40 μL/well). ... (Beyotime, P0013) buffer with protease and phosphatase inhibitor cocktail (Beyotime, P1050). The protein concentration was determined with Pierce™ BCA Protein Assay Kit (Thermo Fisher, …

WebNov 15, 2024 · The detection principle is that a sandwich complex of CSFV E2 – rabbit anti-CSFV E2 antibody – alkaline phosphatase (AP) conjugated goat anti-rabbit IgG formed …

WebLane 1: Recombinant CSFV E2 protein (200ng) (bs-20116P). Probed with CSFV E2 polyclonal Antibody, unconjugated (bs-4527R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C. The immunogen for this antibody is sourced strain Brescia. can a memory foam mattress be cutWebMar 15, 2024 · A series of truncated and overlapping fragments of CSFV E2, shown in Fig. 3, were designed in this study, and the related primers were listed in Table 1.These Truncated E2 genes were cloned into the expression vector pET-30a. After confirmation in DNA sequencing, positive plasmids were transformed into Escherichia coli BL21 (DE3) … can a parallelogram have obtuse anglesWebMar 21, 2024 · Additionally, we showed that compound C or shPRKAA not only reduced the level of E2 in CSFV-infected host cells (Figure 4C, D,H) ... (P0013) containing a protease/phosphatase inhibitor cocktail (Beyotime, P1051), 0.01 mol/L DTT, and 1 mM PMSF (Beyotime, ST506). For each sample, 200 µl of lysate was incubated with 2 µg of … can a person\u0027s dna be altered or changedWeb(9) Other molecules also have anti-CSFV effects, such as Prostaglandin A1 [120,121], the phage-displayed E2-binding peptides [122], ceramide (C6) (activator of the protein phosphatase 1 pathway ... can a person have shingles internallyWebSynthetic, persistent RNA vectors for controlled expression of one or more heterologous polynucleotide sequences, each of the one or more heterologous polynucleotide sequences enc can a peptic ulcer heal on its ownWebFeb 4, 2024 · A novel CSFV E2 sequence (E2ZJ) was identified from an epidemic strain of Zhejiang for outstanding secretion in baculovirus and enhanced immunogenicity. E2 … can a pilonidal cyst go away on its ownWebApr 22, 2024 · Classical swine fever (CSF) is one of the most important viral diseases of swine worldwide. Although live or attenuated virus vaccines have been used to control … can a pinched nerve cause pins and needles