Cytotoxicity of pei

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August undefined, 2024

WebMar 28, 2024 · Thus, PDA NPs had been conjugated with polyethyleneimines (PEI) of different molecular weight (Mw) to form efficient gene transfection agents, which had abundant positive charges to efficiently bind with anionic plasmid ... The cytotoxicity on RAW 264.7, chondrocytes and osteoblasts were detected through CCK- 8 assay. As … WebNov 8, 2002 · To determine whether the lower expression of PEI-complexed DNA at the N/P ratio of 20/1 than the 10/1 N/P ratio is due to the cytotoxicity of PEI, we performed the MTT assay for cell viability.

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Web1 day ago · Binary and ternary polyplexes with PEI/DNA ratio of 1:1 (w:w), as well as free PEI at an equivalent concentration, exhibited minimal cytotoxicity, regardless of the presence of PGA. Free PEI at concentrations equivalent to that present in the polyplexes with PEI/DNA 3:1 and 5:1 (w:w), however, decreased viability by 20–30% and ∼40–60% ... WebFeb 1, 2007 · The results have shown that PSS-ended substrates appear to be the most suitable to drive the cell adhesion and phenotype maintenance of MSCs, showing good … great lakes new holland mitchell

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WebAug 1, 2024 · The cellular toxicity of PEI molecules depends on their structure, molecular weight and surface charge density. To improve the properties of branched 25 kDa PEI as a non-viral gene delivery agent, … WebJun 30, 2015 · Therefore, it seems that PEI could induce cell death through induction of apoptosis process. 21 The purpose of this study was to separate and recognize the … WebCell culture and cytotoxicity assay SH-SY5Y human neuroblastoma cells (C611, NCBI, Tehran, Iran) were incubated at 37°C in a humidified (100% humidity) atmosphere of 5% CO 2 in 100 μL DMEM (1 g/L glucose, 2 mM glutamine) supplemented with 10% FBS, 100 μg/mL streptomycin, and 100 IU/mL penicillin. great lakes navy training facility

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WebFeb 1, 2007 · The cytotoxicity of PEI is known to be molecular weight-dependent. Morimoto's work showed that high molecular weight of PEI (70 kDa) is more cytotoxic … WebMay 25, 2024 · Cytotoxicity of PEI, WSLP, and of WSLP/pDNA is evaluated on human A549 and HeLa cells. A remarkable dependence of the toxicity on the dose, cholesterylation, and charge ratio is detected. Transfection is monitored by flow cytometry and by fluorescence microscopy. floaty mouseWebJun 1, 2005 · PEI is also cytotoxic, but the molecular basis of its cytotoxicity is poorly understood. Here, we have demonstrated that branched (25 kDa) and linear (750 kDa) PEI can both induce membrane damage and initiate apoptosis in three clinically relevant … floaty names

"WebThe different PEI compositions provide various properties and reactivities to particular structures. 21 In addition, the molecular weight of PEI varies broadly, and high-molecular-weight-PEI is associated with increased cytotoxicity. Also, the effects of PEI on DNA damage are considered based on the polymer/DNA (C/P) ratios. " - Cytotoxicity of pei

Cytotoxicity of pei

A two-stage poly(ethylenimine)-mediated cytotoxicity: implications for …

WebPoly(ethylenimine) (PEI) is a cationic macromolecule commonly used in gene transfer/therapy protocols with high transfection efficiency both in vitro and in vivo. PEI is also cytotoxic, but the molecular basis of its …

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WebMar 1, 2014 · PEI has shown relatively low cytotoxicity when complexed with DNA and also higher transfection ef ficiencies significantly better than those observed with PLL … WebPEI is also cytotoxic, but the molecular basis of its cytotoxicity is poorly understood. Here, we have demonstrated that branched (25 kDa) and linear (750 kDa) PEI can both induce …

WebJul 14, 2009 · Also, cytotoxicity studies on MAT-LyLu and MBT cell lines were performed with QA-PEI nanoparticles. These findings confirm previous reports that polycations bearing quaternary ammonium moieties inhibit bacterial growth in vitro and have a potential use as additives in medical devices which need antibacterial properties. 1. Introduction WebApr 13, 2024 · PEI, with high molecular weight, possesses better transfection ability. For example, 25 kDa PEI is considered as the “gold standard” effective transfection reagent . However, the higher the molecular weight, the higher the cytotoxicity of PEI. Therefore, many PEI derivatives have been developed to reduce cytotoxicity without affecting the ...

WebSep 24, 2012 · The transfection efficiency and the cytotoxicity of PEI are closely linked to its structural activity. This structural relationship is an essential issue to be investigated in detail in order to show the effect of chemical structure, hydrophobicity, charge density, and molecular weight of PEI on its transfection activity as well as cytotoxicity Web2.6. In vitro cytotoxicity: Cytotoxicities of PEI/pDNA, PEG (10)–PEI/pDNA, PEG(20)–PEI/pDNA and PEG(30)–PEI/pDNA polyplexes were evaluated on BT-474 and …

WebJun 30, 2024 · The cytotoxicity of PEI-HMSNs was found to be concentration-dependent ( Figure 4 D). However, the cell viability of PEI-HMSNs was still found to be higher than 85% when its concentration was 120 µg/mL as shown in Figure 4 D. 2.4. Gene Transfer Efficiency and Cellular Uptake

WebFeb 1, 2024 · Polyethylenimine (PEI)-coated IONPs exhibited significantly higher uptake than PEGylated ones in both macrophages and cancer cells, and caused severe cytotoxicity through multiple mechanisms... great lakes neurosurgical grand rapids miWebIn our previous experiments, we showed that gene transfection efficiency was enhanced by low-dose 25 kDa-branched PEI with the assistance of 1.8 kDa-branched PEI with low cytotoxicity in vitro. 8 To overcome these obstructions, many researches have modified the surface of polymer nanoparticles to achieve safer and more efficient gene delivery ... floaty mother of bride outfits scotlandWebJul 10, 2015 · Transfection efficiency was strongly correlated to PEI cytotoxicity. Also, some evidence for PEI-induced apoptosis in both cell lines was found. In conclusion, our results indicate that PEI is a useful vector for nonviral transfection in … great lakes neuropsychology edinaWebDec 9, 2016 · The polymer OTMCS-PEI-R13 has showed low cytotoxicity as OTMCS-PEI, and higher transfection in vitro. With the following in vivo studies, gene transfection efficiency was found to be much lower compared with in vitro. The main reason is that the transport manner of complexes across nucleus membrane in vivo is different from in vitro. float your boat carlingfordWebCompared with PEI/DNA, the complex vector (PEI/chitosan/DNA with chitosan/DNA N/P=4, PEI/DNA N/P=10) appeared to have low cytotoxicity, which maintained the cell survival rate at greater than 80%, and showed higher transfection efficiency of nearly 1000 fold compared with that using chitosan/DNA alone. floatyourboat.co.ukWebFeb 1, 2024 · We speculate that the cytotoxicity of PEI-coated IONPs may originate from the PEI coating material, which was reported to be cytotoxic via two different … float your boat discount codeWebBased on their high surface charge, PEIs are promising candidates for delivery of negatively charged nucleic acids 17, 18. In general, the transfection capability and cytotoxicity increases with the increase of molecular weight 4. Linear PEI shows lower cytotoxicity and higher efficiency than branched PEI for gene transfection 19, 20. great lakes new holland st mary\u0027s