Jc 1 staining principle
WebJan 1, 2024 · JC-1 can be used to assess the ΔΨ M both in intact isolated mitochondria and tissues. In fact, the JC-1 dye is a lipophilic, cationic dye (naturally exhibiting green … WebJC-1) to green fluorescence (JC-1 monomers). This kit enables a fast and simple staining of isolated mitochondria from animal tissues and cell lines. It includes valinomycin, a mitochondrial membrane-dissipating agent, for control experiments. Components The kit is sufficient for 50 staining assays of 2 ml using JC-1 Stain 25 g
Jc 1 staining principle
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WebThe stain protocol is simple and straightforward: incubate the cells with JC-1 dye and then analyze the fluorescent signal in the FITC and PE channels of of a 488 nm laser. For Research Use Only. Not for use in diagnostic … WebMay 9, 2024 · In the normal state, JC-1 aggregates in the mitochondrial matrix to form polymers, producing red fluorescence signals; when mitochondrial membrane potentials decrease, JC-1 disaggregates to monomers which produce green fluorescence. Assays were conducted as follows.
WebJC-1 is a cationic dye that accumulates in energized mitochondria. In apoptotic cells with low MMP, JC-1 remains in a monomeric form, which exhibits green fluorescence. While in … WebAll staining procedures must be performed without direct exposure to intense light, as JC-1 is light sensitive. If staining is too intense, bring down the final JC-1 concentration may...
WebThe membrane-permeant JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health. JC-1 dye can be used as an indicator of mitochondrial membrane potential in a variety of cell types, including myocytes and neurons, as well as in intact … JC-1 is a novel cationic carbocyanine dye that accumulates in mitochondria. The … JC-1 dye exhibits potential-dependent accumulation in mitochondria, indicated … WebStaining the Cells Note Protect the JC-1 reagent from the light at all times. 1. Following induction for the required activation time, transfer 500 μl, containing 1 × 106 induced cells, into a sterile centrifuge tube. Centrifuge the cell suspension for 5 minutes at 400 × g at room
WebNov 22, 2012 · The cyanine dye JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimi- dazolylcarbocyanine iodide) facilitates discrimination of energized and deenergized …
Web(B) MitoProbe JC-1 Assay Kit was used to stain Jurkat cells (T-cell leukemia, human) which were then analyzed on a flow cytometer using 488 nm excitation with 530 nm and 585 nm bandpass emission filters. Green = apoptotic cells (reduced mitochondria membrane potential), red = normal cells. TOP MitoProbe DiOC 2 (3) Assay Kit nottingham trent fashion coursesWeb1,1’,3,3’‐tetraethylbenzimi‐dazoylcarbocyanine iodide (JC‐1). In healthy cells, JC‐1 enters the energized mitochondria and form aggregates which change the fluorescent property of … nottingham trent fashion designWebAug 1, 2011 · 2011 Aug 1;2011(8):990-2. doi: 10.1101/pdb.prot5648. Author Brad Chazotte. PMID: 21807856 DOI: 10.1101/pdb.prot5648 Abstract INTRODUCTION Membrane-potential-dependent dyes such as Rhodamine 123, tetramethylrhodamine methyl ester (TMRM), and tetramethylrhodamine ethyl ester (TMRE) are useful as long as the mitochondrion … how to show date on apple watchWebFurthermore, another kind of dye, JC-1, yields green or red fluorescence depending on MMP and allows to distinguish cells with high or low mitochondria potential. Both measuring … how to show declined meetings in teamsWeb1.3 1 mM JC-1 Stock Solution: Allow the lyophilized vial JC-1 and the DMSO to warm to room temperature. Reconstitute the JC-1 by adding 766.8 µL DMSO to the 500 µg vial of JC-1. Any unused 1 mM JC-1 solution should be aliquoted and stored at -20 ºC. 1.4 Working JC-1 Solution: To prepare the Working JC-1 Solution, add the appropriate volume how to show dedication at workWebDec 26, 2015 · 5. Wash each staining jar with three (3) changes of 1% Calcium Chloride for a total of approximately ten (10) minutes. 6. Add 2% Cobalt Chloride to each jar for ten (10) minutes. 7. Wash with three (3) to five (5) changes of an approximately 1:20 solution of 0.1M Sodium Barbital (~ 5 ml 0. 1 M Sodium Barbital + deionized water ~ 100 ml). nottingham trent fashion managementWebJun 1, 1998 · The staining intensity of JC-1 in HSC reverted to the levels seen in unseparated bone marrow samples when the staining was performed in the presence of the MDR blockers, suggesting preferential efflux of JC-1 by an MDR mechanism in HSC. nottingham trent cricket