Mitotimer ミトコンドリア

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August undefined, 2024

Web(A) heK 293 cells were transfected with pTRe-MitoTimer (MT), exposed to Dox (+) or vehicle (−) for 24 h, then subjected to subcellular fractionation to obtain mitochondria … WebMitoTimer as a tool for monitoring mitochondrial turnover in vitro and in vivo. 3. Results and discussion 3.1. Constitutive expression of MitoTimer in vitro High membrane potential is required for mitochondrial protein import; thus hypo-polarized mitochondria cannot import newly syn-thesized (green) MitoTimer protein and consequently exhibit a high

MitoTimer ReporterGeneforMitochondrialContent, …

WebOct 21, 2015 · Furthermore, individual mitochondria within cardiomyocytes display a higher red-to-green fluorescence, implying a block in import of newly synthesized MitoTimer … WebJan 31, 2024 · We demonstrate that the fluorescent protein, MitoTimer, is a reliable and robust probe to follow mitochondrial turnover. The screening of 15 000 small molecules led us to two chemically-related benzothiophenes that stimulate basal mitophagy in the beta-cell line, INS1. Enhancing basal mitophagy was associated with improved mitochondrial ... i am fine with both

Conditional MitoTimer reporter mice for assessment of …

WebJan 1, 2014 · The MitoTimer fluorescent protein has been described in recent studies as a new probe to measure different aspects of mitochondrial turnover and efficacy of quality control ( Ferree et al., 2013, Hernandez et al., 2013, Laker et al., 2014). MitoTimer consists of the Timer fluorescent protein fused to the mitochondrial targeting sequence of ... WebMay 21, 2024 · To directly monitor mitochondrial turnover, MitoTimer labeling was used 30. MitoTimer is a mitochondria-targeting florescence protein that is green once being … WebDec 6, 2014 · MitoTimer also provides information about the level of mitochondrial protein import across a population of cells and organelles. High membrane potential is required … momentsinthyme.com

MitoTimer localizes to mitochondria. (A) heK 293 cells

In vivo assay and modelling of protein and mitochondrial turnover ...

WebMitotimer showed mitochondrial localization and changed in color from green to red over time. Scale bar: 20 µm. (B) Primary hippocampal neurons were transfected and imaged at the different time points after doxycycline induction. MitoTimer showed similar localization to mitochondria and kinetics of green to red transition as in MEF cells. WebmitoTimer体系：【将Timer基因与线粒体靶向序列结合，获得的MitoTimer蛋白能够在线粒体富集，实现对线粒体状态的检测。 Timer蛋白是红色荧光蛋白dsRed的突变体，其荧光 … moments in spacetime movieWebMay 1, 2016 · The MitoTimer transgene combines the red fluorescent protein, dsRed, and a human cytochrome c oxidase subunit VIII (Cox 8) mitochondrial localization sequence to traffic the protein to the mitochondria where the color shifts from green to red over time (approximately 24 hours). Our goal is to use this as a tool to assess the increase or ... moments in the woods sheet music pdf free

"WebOct 11, 2013 · MitoTimer was detected in the mitochondrial fraction but absent in the cytosolic fraction, demonstrating specificity of mitochondrial targeting. In the absence of … " - Mitotimer ミトコンドリア

Mitotimer ミトコンドリア

Abstract 89: MitoTimer Mouse: a Novel Tool for the Study of ...

WebDec 1, 2015 · MitoTimer cDNA was introduced by blunt 5'end of the insert and the NheI-compatible site XbaI, ligated into a Mung Bean nuclease-blunted SalI site and the NheI site of the vector. MitoTimer transgenic mice were created in the C57/BL6 strain (Jackson Laboratories, Sacramento, CA) by pronuclear injection of murine alpha myosin heavy … WebMitoTimer: A novel tool for monitoring mitochondrial turnover. Hernandez G, Thornton C, Stotland A, Lui D, Sin J, Ramil J, Magee N, Andres A, Quarato G, Carreira RS ...

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WebMitoTimer: A novel tool for monitoring mitochondrial turnover. Hernandez G, Thornton C, Stotland A, Lui D, Sin J, Ramil J, Magee N, Andres A, Quarato G, Carreira RS ... WebMitoTimer expression was detected as early as 4 h and persisted (in the presence of continuous Dox) for at least 72 h. To overcome the asynchronous incorporation of …

WebMar 18, 2014 · Cox4 enrichment in the mitochondrial fraction and Gapdh in the cytosolic fraction indicate successful cell fractionation. d, expression of MitoTimer in a C. elegans touch neuron with mitochondria ... WebPlasmid pTRE-Tight-MitoTimer from Dr. Roberta Gottlieb's lab contains the insert MitoTimer and is published in Autophagy. 2013 Nov 1;9(11):1852-61. doi: …

Web文献「MitoTimerは若いおよび老いたミトコンドリア蛋白質の細胞内分布および相対的ミトコンドリア蛋白質年齢に対するオートファジー,融合および運動能の効果を明らかにす … WebMitoTimer is a mutant of the red fluorescent protein DsRed, in which the fluorescence shifts irreversibly from green to red as the protein is oxidized. They targeted the probe to the mitochondrial matrix with a mitochondrial-targeting sequence. MitoTimer can continuously monitor mitochondrial dynamics within cells and is of particular value ...

WebMay 21, 2024 · To directly monitor mitochondrial turnover, MitoTimer labeling was used 30. MitoTimer is a mitochondria-targeting florescence protein that is green once being translated and becomes more red over ...

WebMitoTimer transgenic fly line, the coding region of MitoTimer wassubclonedintopUASvector.AUAS-MitoTimertransgenic fly line was established commercially (Genetic Services, Inc.). UAS-MitoTimer flies were crossed with Mef2-Gal4 to obtain Mef2-Gal4UAS-MitoTimer flies.Adultfemaleflieswerecol-lected under light CO 2 … moments in tamaracWebConclusion: MitoTimer is useful for assessment of mitochondrial health in vivo. Significance: MitoTimer could advance mitochondrial research in multiple disciplines. Mitochondrial … i am fine with anytimeWebOct 11, 2013 · MitoTimer is a novel fluorescent reporter protein that can reveal new insights into mitochondrial dynamics within cells. Coupled with organelle flow cytometry, it offers … moments in the sky blogsWebOct 11, 2013 · Flow cytometry analysis of MitoTimer in cells. ( A) Transfected cells were exposed to Dox for 1 h, then cultured for the indicated times (4–144 h), harvested, and … i am fine with either oneWebDec 14, 2024 · MitoTimer is a fluorescent protein targeted to the mitochondrial matrix. Upon translation, it emits green fluorescence, but after ∼16 h its emission shifts to red. Thus, the red: green ratio of cells expressing MitoTimer serves as an indicator for mitochondrial turnover, with an increase in the red: green ratio demonstrating a reduction in ... i am fine with it synonymWeb(A) heK 293 cells were transfected with pTRe-MitoTimer (MT), exposed to Dox (+) or vehicle (−) for 24 h, then subjected to subcellular fractionation to obtain mitochondria (lanes 1-3) and ... i am fine with either dayWebDec 6, 2014 · MitoTimer also provides information about the level of mitochondrial protein import across a population of cells and organelles. High membrane potential is required for mitochondrial protein import . Mitochondria which lose membrane potential will be unable to import new (green) MitoTimer protein. If biogenesis is impaired, one would expect a ... moments in time artwork