Photoblueing
WebFeb 26, 2024 · Its fluorescence has been shifted towards the blue range on the wavelength scale. This is why this effect is called photoblueing,“ explains Professor Markus Sauer, an expert in super-resolution microscopy from the Biocentre of Julius-Maximilians-Universität Würzburg (JMU) in Bavaria, Germany. First exact description of photoblueing WebMar 1, 2024 · Mar 01, 2024: How photoblueing disturbs microscopy (Nanowerk News) The latest developments in fluorescence microscopy make it possible to image individual …
Photoblueing
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WebFeb 25, 2024 · Photoblueing of organic dyes can cause artifacts in super-resolution microscopy. Dominic A Helmerich Department of Biotechnology and Biophysics, … WebJan 16, 2024 · Affiliations. 1 Department of NanoBiophotonics , Max Planck Institute for Biophysical Chemistry , Am Fassberg 11 , 37077 Göttingen , Germany. 2 Department of …
Webprevent photoblueing by simple additives such as vitamin C or to increase it by adding a kind of catalyst," says Markus Sauer. Preventing photoblueing can be quite important. … WebPhotoblueing of organic dyes can cause artifacts in super-resolution microscopy Dominic A. Helmerich et al., 2024, Nature Methods. Staining MIF in Cells for Confocal Microscopy James Harris, 2024, Springer Protocols. High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the …
WebFeb 26, 2024 · How Photoblueing Disturbs Microscopy. An undesirable effect can occur in super-resolution fluorescence microscopy: photoblueing. A new publication in „Nature … The dyes Alexa Fluor 647 (LifeTech, A20106), Cy5 (GE-Healthcare, PA15101), ATTO647N (ATTO-TEC, AD647N-31), CF660C (GE-Healthcare, SCJ4600050), ATTO565 (ATTO-TEC, AD565-31), and a custom-modified goat anti-rabbit IgG secondary antibody (Invitrogen, 31212) labeled with Alexa Fluor … See more Steady-state absorption and fluorescence emission spectra were recorded on a V-650 spectrophotometer (Jasco) and an FP-6500 spectrofluorometer (Jasco). Samples were … See more The photobleaching efficiency of fluorophores was analyzed at the ensemble level in a 0.3 mm path-length fluorescence cuvette (Hellma, 105.251-QS) using 10 µM dye solutions in PBS (Sigma, D8537-500ML), with … See more Fluorescence lifetime measurements were performed on a MicroTime200 (PicoQuant) time-resolved confocal fluorescence microscope setup consisting of a FLIMbee galvo … See more For analysis of the fluorescence lifetime of photoconverted Cy5 (GE-Healthcare, PA15101), the photobleached solution was purified using HPLC on a Kinetex biphenyl column (150 × 4.6 mm), consisting of 2.6 μm … See more
Webwidespread use of the cyanine scaffold, the chemistry underpinning this “photoblueing” artifact had remained opaque. We recently found that the irradiation of the simplest pentamethine cyanine, 2, leads to formation of the corresponding trimethine cyanine, 3 (Fig. 1b).13 This reaction, involving the formal excision of ethene diradical from ...
WebMar 1, 2024 · Here, we discuss the mechanisms of photoblueing of fluorophores and its impact on fluorescence imaging, and show how it can be prevented. Dominic A. Helmerich, Gerti Beliu, Siddharth S. Matikonda ... perrysburg wrestling tournamentWebFeb 26, 2024 · An undesirable effect can occur in super-resolution fluorescence microscopy: photoblueing. A new publication in „Nature Methods” shows how it can be prevented or made useful for research. Confocal fluorescence images of glass surfaces coated with the cyanine dyes Alexa Fluor 647 (a) and CF660C (b) and with carborhodamine dye … perrysburg wrestlingWebFirst exact description of photoblueing Sauer's team now presents the exact molecular mechanism of photoblueing for cyanine dyes like Cy5 for the first time in the journal … perrysburg wrestling clubWebMar 1, 2024 · The first exact description of photoblueing. For the first time, Sauer’s research team has now presented the precise molecular process of photoblueing for cyanine dyes, … perrysburg wrestling scheduleWebPhotoblueing of organic dyes can cause artifacts in super-resolution microscopy. 25 February 2024. Dominic A. Helmerich, Gerti Beliu, … Markus Sauer. Structural colors of pearls. perrysburg yellow jackets footballWebFeb 25, 2024 · Here, we discuss the mechanisms of photoblueing of fluorophores and its impact on fluorescence imaging, and show how it can be prevented. Full text links . Read article at publisher's site (DOI): 10.1038/s41592-021-01061-2. Citations & impact . This article has not been cited yet. perrysburg yellow jacketsWebOct 1, 2006 · Photoblueing of organic dyes can cause artifacts in super-resolution microscopy. 25 February 2024. Dominic A. Helmerich, Gerti Beliu, … Markus Sauer. perrysburg ymca gymnastics