WebJun 3, 2024 · We develop CRISPReader, a technology for controlling promoterless gene expression in a robust fashion. We demonstrate that this tool is highly efficient in controlling transcription and translation initiation of a targeted transgene. ... Reported data are the mean ± SD from five experiments. **P < 0.01, compared with the sgRNA-negative control ... WebJul 25, 2007 · To ensure efficient expression of the gfp gene in R. erythropolis from the tested promoters, the promoterless gene gfpuv, with 5′ end fusion with the initial six codons of the aph gene and upstream insertion of the aph Shine-Dalgarno sequence, was used as a reporter gene in the promoter-probe vector pEPR1. ... The mean values (fluorescence in ...
promoterless - English definition, grammar, pronunciation, …
WebFeb 13, 2024 · Promoterless definition: Collins Dictionary Definition Meaning, pronunciation, translations and examples WebLearn the definition of 'promoterless'. Check out the pronunciation, synonyms and grammar. Browse the use examples 'promoterless' in the great English corpus. duke primary care internal medicine
Gene targeting using a promoterless gene trap vector ... - PNAS
WebNational Center for Biotechnology Information WebTrapping is performed with gene trap vectors whose principal element is a gene trapping cassette consisting of a promoterless reporter gene and/or selectable genetic marker, … WebApr 12, 2024 · We firstly established a promoterless EGFP reporter donor targeting the porcine GAPDH locus to study CRISPR/Cas9-mediated homologous recombination in porcine cells. Curiously, EGFP was expressed at unexpectedly high levels from the promoterless donor in porcine cells, with or without Cas9/sgRNA. duke primary care in durham