Slowfade diamond antifade mountant
WebbProLong Diamond Antifade Mountant with DAPI provides first-class photobleach protection for Alexa Fluor fluorescent dyes and infrared proteins with nuclear DNA … Webb14 feb. 2024 · Tissues were washed three more times and stained with 1X DAPI (Cat# 62248; Thermo Fisher) in TBS for 10 min and mounted on microscope slides with cover slips and SlowFade TM Gold Antifade Mountant media (Cat# S36937; Invitrogen).
Slowfade diamond antifade mountant
Did you know?
WebbSlowFade™ Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. SlowFade™ Diamond mountant provides superior … WebbSlowFade Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. It provides …
WebbProLong Gold Antifade Mountant is offered in a ready-to-use 2 mL dropper bottle, or 10 mL bottle and is stored at room temperature. Just apply a drop on the sample, add a coverslip, cure, and image. For high-resolution … WebbSlowFade Diamond reagent is a superior antifade and mountant, offering protection across the visible spectrum and exhibiting less initial quenching of the fluorescent signal. It provides additional protection for traditional …
WebbFor mountants with 1.52 refractive index, optimized for oil immersion objective imaging, try curing mountant Prolong Glass Antifade Mountant or non-curing SlowFade Glass Soft-set Antifade Mountant. ProLong Gold Antifade Mountant comes already mixed and ready to use in a dropper bottle or 10-mL bottle with or without DNA stain. WebbSlowFade Glass antifade reagent is the ideal mountant for specimens with imaging depths from 0-500 µm, including cultured cells, organoids/spheroids, and thin and thick tissue sections. The mountant provides excellent protection against photobleaching across the visible and near infrared spectra, and it can be used with most fluorescent dyes ...
Webb11 maj 2024 · To determine whether Fgfr2 is required for AT2 cell homeostasis in the adult lung, we inactivated Fgfr2 in AT2 cells and lineage-traced these cells in adult mice to assess lung morphometry and AT2 cell fate at three time points: 1 …
WebbFor mountants with 1.52 refractive index, optimized for oil immersion objective imaging, try curing mountant Prolong Glass Antifade Mountant or non-curing SlowFade Glass Soft … photo of isaac newton and the falling appleWebbNuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. photo of israelWebbAfter final washing in PBS, slides were closed in SlowFade™ Diamond Antifade Mountant with 4-6-diamidino-2-phenylindole (DAPI; ThermoFisher Scientific). Sections were imaged by confocal microscopy using a Zeiss Laser Scanning Inverted Microscope LSM-700 equipped with 40X/1.3 Oil NA objective and Black Zen software (Carl Zeiss) at the Center … photo of isabellaWebbProLong Adamant Antifade Mountant with DAPI provides superior photobleach protect for Alexa Fluor fluorescent dyes and fluorescent proteins with nuclear DNA counterstain (DAPI) within an mountant. ProLong™ Diamond Antifade Mountant with DAPI - Protocol to assess the effects of dysfunctional human vascular smooth muscle cells on other brain … photo of island beachWebbCologne, Germany) antibodies を使用した。細胞核の染色は SlowFade™ Diamond Antifade Mountant with DAPI (ThermoFisher, Massachusetts, USA) を用いた。免疫 染色した細胞をスライドガラスに滴下し、15×15㎜カバーガラスで封入して、観察用 スライドを作成した。 画像処理と加工 how does molly affect your brainWebb2 sep. 2024 · Cells were washed three more times with PBST and mounted on slides using SlowFade Diamond Antifade Mountant (Thermofisher Scientific, Cat. #S36972). After allowing to set for 24 hr, cover-slips were sealed in place using clear nail polish. Images were captured using a Zeiss LSM 880 confocal microscope and processed using ImageJ … how does molly affect youWebbLearn about imaging mounting mediums containing an antifade to prevent photobleaching. how does monarch work mtg